Preparation of Polymer Immunoparticles as a Tool for Separation Oxidative Stress Bio¬markers from Body Fluids
a Department of Organic Technology, Institute of Chemical Technology, Prague; b Department of Occupational Medicine, 1st Faculty of Medicine, Charles University in Prague
The work deals with preparation and characterization of polymer immunoparticles used as a separation tool for biomarkers from biological fluids like urine, plasma etc. Polymer imunoparticles consist of antibodies immobilized in a polyvinyl alcohol and polyethylene glycol matrix. Prepared immunoparticles, containing monoclonal antibodies against 8-iso Prostaglandin F2α, were used as a tool for a preliminary separation of 8-iso Prostaglandin F2α (a marker of oxidative stress) from complex biological matrix (urine) before a highly specific and precise detection and quantification by LC-ESI-MS method. The deve¬loped method was characterized by high precision and accuracy. Functionality of the method was tested in a clinical study where urine concentration levels of 8-iso Prostaglandin F2α in patients with diagnosis of asbestosis or silicosis (oxidative stress induced diseases) and control group were compared.
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