Application of Flow Cytometry of Yeast in Research and Practice

  • J. Novák Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Prague
  • G. Basařová Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Prague
  • J. Fiala Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Prague
  • P. Dostálek Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Prague

Abstrakt

Flow cytometry as a rapid technique can be used for monitoring the yeast (Saccharomyces cerevisiae) growth in biotechnological processes. The technique permits analysis of single cells in suspension and thus to obtain cell size distribution, granularity, intracellular contents of DNA, proteins, and sterols, viability, cell cycle, intracellular pH and enzyme activities. Flow cytometry can also be used for cell sorting. Despite the advantages of flow cytometry, implementation of the technique in biotechnological laboratories is still limited.
Publikované
2008-04-15
Sekce
Články