Hodnocení aktivity reaktivních sorbentů pomocí standardního fosfatasového testu

  • N. Zemanová Univerzita Jana Evangelisty Purkyně, Fakulta životního prostředí, Ústí nad Labem
  • M. Kunešová Univerzita Jana Evangelisty Purkyně, Fakulta životního prostředí, Ústí nad Labem
  • V. Pilařová Univerzita Jana Evangelisty Purkyně, Fakulta životního prostředí, Ústí nad Labem
Klíčová slova: alkalická fosfatasa, fosfatasová aktivita, oxidy kovů, nanočástice, reaktivní sorbenty

Abstrakt

Since recently, nanoparticles of certain metal oxides (e.g. oxides of Ti, Ce, Fe), the so called reactive sorbents, have been used for absorption or for decomposition of hazardous chemicals. The present article deals with the assessment of the phosphatase-mimetic activity of reactive sorbents made for decomposition of dangerous organophosphorus compounds such as pesticides or nerve gases. The aim was to find a quick, simple and robust method for comparing the phosphatase activity of these reactive sorbents. For this purpose, a standard and commercially available test was selected. This test is used for determination of the catalytic concentration of alkaline phosphatase in blood serum (ALP-MEG L 500, Erba Lachema, Brno, Czech Republic). The test was modified and optimized for the evaluation of phosphatase-mimetic activity of various types of reactive sorbents under static or kinetic conditions. Both methods were validated and the analytical characteristics discussed.

Publikované
2016-03-15
Sekce
Články