Determination of Phenolic Substances in Plant Materials by Capillary Electrophoresis and Liquid Chromatography

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  • J. Vlcek Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, 613 00 Brno
  • B. Klejdus Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, 613 00 Brno
  • V. Kuban Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, 613 00 Brno

Abstrakt

A simple, sensitive and fast capillary electrophoretic method was optimized for the identification and determination of allelopathic phenolic compounds (vanillin, caffeic, 4-hydroxybenzoic, ferulic, salicylic, protocatechuic and 4-coumaric acids) in plant materials. Separation in an uncoated silica capillary (Supelco Select - FS75, effective length 22 cm, total length 31 cm, 75 mm I.D., 363 mm O.D.) was performed at 6 kV in 20 mmol/l boric acid and 60 mmol/l Na2HPO4 buffer (pH 8.75). The method was applied to the determination of vanillin, 4-hydroxybenzoic and protocatechuic acids in Ranunculus bulbosus, Veronica chamaedrys and Sanguisorba minor and in barley seeds. The results were in good agreement with those obtained by liquid chromatography.

Publikováno

15.02.2002

Jak citovat

Vlcek, J., Klejdus, B., & Kuban, V. (2002). Determination of Phenolic Substances in Plant Materials by Capillary Electrophoresis and Liquid Chromatography. Chemické Listy, 96(1). Získáno z http://www.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2357

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