Utilization of Ultra Performance Liquid Chromatography (UPLC) – Mo¬dern Separation Technique for Determination of Vitamin E in Barley Cereals

  • K. Benešová Malting Institute Brno,
  • H. Pluháčková Mendel University Brno, Czech Republic,
  • S. Běláková Malting Institute Brno,
  • K. Vaculová Agricultural Research Institute, Kroměříž, Czech Republic
  • R. Mikulíková Malting Institute Brno,
  • J. Ehrenbergerová Mendel University Brno, Czech Republic,
  • N. Březinová Belcredi Mendel University Brno, Czech Republic,

Abstrakt

A new UPLC method for the determination of vitamin E in barley cereals is proposed. The method shows higher sensitivity and lower limits of detection and quantification than the previous HPLC methods. The method was applied to various spring barley varieties. Although it was not possible to separate all vitamin E isomers due to the reverse phase used, the rapid and inexpensive method is suitable for a long-term monitoring of vitamin E in barley and other cereals.
Publikované
2012-08-15
Sekce
Články