Chromatographic Analysis of Naphthoquinones in Plants

  • P. Babula Department of Natural Drugs, University of Veterinary and Pharmaceutical Sciences,
  • R. Mikelová Department of Chemistry and Biochemistry,
  • V. Adam Department of Chemistry and Biochemistry,
  • D. Potěšil Department of Chemistry and Biochemistry,
  • J. Zehnálek Department of Chemistry and Biochemistry,
  • R. Kizek Department of Chemistry and Biochemistry,
  • L. Havel Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Brno
  • Z. Sladký Department of Natural Drugs, University of Veterinary and Pharmaceutical Sciences,

Abstrakt

The aim of the work was to optimize the high performance liquid chromatography coupled with a diode array detector for the determination of naphthoquinones (1,4-naphthoquinone, lawsone; juglone and plumbagin). The optimal conditions were: mobile phase 0.1 mol l-1 acetic acid – methanol (35 : 65 v/v), flow rate 0.8 ml min-1, column and detector temperature 40 °C. Detection limits (3 S/N) were 65 ng ml-1, 50 ng ml-1, 75 ng ml-1 and 39 ng ml-1 for lawsone, 1,4-naph¬tho¬qui¬none, juglone and plumbagin, respectively. The optimized method was used for the determination of the mentioned naphthoquinones in plants (Dionaea muscipula, Drosera rotundifolia, Drosera spathulata, Drosera capensis and Paulownia tomentosa).
Publikované
2006-05-15
Sekce
Články