Epitachophoresis – A Method for the Extraction of Biomacromolecules from Complex Biological Matrices

New Perspectives on Analytical Chemistry

Abstract

Epitachophoresis is a new separation preparative technique based on discontinuous electrolyte systems enabling the preconcentration of biomacromolecules from complex biological matrices. Moreover, it enables the transfer of these compounds from biological liquids, such as urine or growth medium, to a simpler solution, suitable for subsequent analyses. In our recently published papers, we focused on the use of epitachophoresis to pretreat the samples containing proteins and DNA. The technique enabled more than seventyfold preconcentration with recoveries reaching more than 90%. The preparative nature of epitachophoresis enables one to subsequently analyse the isolated biomacromolecules by various techniques including mass spectrometry, capillary electrophoresis, gel electrophoresis, ELISA analysis, or new-generation sequencing.

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