Application of Hydrophilic Interaction Liquid Chromatography in Separation of Polar Compounds

  • J. Vacek Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • L. Onofrejová Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • B. Klejdus Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • V. Kubáň Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno

Abstrakt

Applicability of classical procedures based on reverse-phase liquid chromatography in separation of water-soluble polar substances is generally limited. In many cases, hydrophilic interaction liquid chromatography (HILIC) is able to separate highly polar substances using polar stationary phases and aqueous mobile phases containing increased amounts of organic solvents. Separation of amino acids, peptides, nucleic acids and other organic substances with special emphasis on explanation of basic principles are discussed in the present review.
Publikované
2009-06-15
Sekce
Články