Application of Fluorimetric Determination of Esterases in Plant Material

  • J. Víteček Department of Plant Biology and Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • V. Adam Department of Plant Biology and Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • J. Petřek Department of Plant Biology and Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • P. Babula Department of Plant Biology and Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • P. Novotná Department of Plant Biology and Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • R. Kizek Department of Plant Biology and Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno
  • L. Havel Department of Plant Biology and Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Brno

Abstrakt

Esterases show a great potential in plant biotechnology. In this article we report a simple method for assessment of their activity using fluorescein diacetate. The method was applied to cell suspension cultures of tobacco BY-2 line, a culture of early somatic embryos of Norway spruce on a semisolid medium and to a suspension culture of onion. We demonstrated that the intracellular esterases can be used for construction of growth curves of the above mentioned cultures. In addition, we tested the method on suspension cultures of onion and bacteria.
Publikované
2005-08-15
Sekce
Články