Evaluation of the ETA-AAS and HG-AAS Methods of Selenium Determination in Vegetables

  • O. Hegedüs Research Institute of Vegetables, Nové Zámky,
  • A. Hegedüsová Department of Chemistry, Constantine the Philosopher University in Nitra, Slovak Republic
  • J. Gašparík Regional Authority of Public Health, Nové Zámky,
  • A. Ivičičová Regional Authority of Public Health, Nové Zámky,


Two methods of selenium determination, ETA-AAS and HG-AAS, were compared and evaluated. The following validation parameters were determined: accuracy (under repeatability conditions), trueness, calibration curve and linearity, limit of detection, limit of determination and combined standard uncertainty of the method. The HG-AAS method was more suitable for the determination of low selenium concentrations in vegetables. The results were calculated by the standard addition method because of the strong matrix effect.