Utilizing Electrochemical Techniques for Detection of Biological Samples

  • O. Zítka Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno,
  • K. Stejskal Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno,
  • A. Kleckerová Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno,
  • V. Adam Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno,
  • M. Beklová Department of Veterinary Ecology and Environmental Protection, University of Veterinary and Pharmaceutical Sciences, Brno,
  • A. Horna Radanal Ltd., Pardubice,
  • V. Šupálková Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno,
  • L. Havel Department of Plant Biology, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno,
  • R. Kizek Department of Food Engineering, Faculty of Technology, Zlín

Abstrakt

Heavy metals rank among the most toxic compounds occurring in the environment; they are also dangerous due to bioaccumulation. Plants and animals have developed a number of protective mechanisms. The detoxification mechanisms of heavy metals in different organisms have been intensively studied for many years. We aimed at investigation of detoxification mechanisms of maize plants treated with 0, 50, 100, 150, 200, 400 and 500 μM Cd(II) solutions for six days. In particular, we observed their growth and determined the Cd content (by differential pulse anodic stripping voltammetry) and thiol concentration (by HPLC) in the treated plants. Maize plants took up 6 pg of Cd per gram per hour at the lowest dose and 23 pg Cd per g per hour at the highest Cd dose. The relations of glutathione and phytochelatin contents, applied Cd dose, cultivation time, growth curve and plant morphology were investigated.
Publikované
2007-04-15
Sekce
Články

Nejaktuálnější články stejného autora (stejných autorů)